Canopy temperatures are important for understanding tree physiology, ecology, and their cooling potential, which provides a valuable ecosystem service, especially in urban environments. Linkages between tree species composition in forest stands and air temperatures remain challenging to quantify, as the establishment and maintenance of onsite sensor networks is time-consuming and costly. Remotely-sensed land surface temperature (LST) observations can potentially acquire spatially distributed crown temperature data more efficiently. We analyzed how tree species modify canopy air temperature at an urban floodplain forest (Leipzig, Germany) site equipped with a detailed onsite sensor network, and explored whether mono-temporal thermal remote sensing observations (August, 2016) at different spatial scales could be used to model air temperatures at the tree crown level. Based on the sensor-network data, we found interspecific differences in summer air temperature to vary temporally and spatially, with mean differences between coldest and warmest tree species of 1 textasciicircum